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pcr amplicon product  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcr amplicon product
    Pcr Amplicon Product, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+product/Agarose+LE%2C+Molecular+Biology+Grade%2C+Ultrapure/pm42321340-238-2-14
    Average 97 stars, based on 1 article reviews
    pcr amplicon product - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Molecular Identification of Spirogyra Species Using rbcL Markers and Their Chemical Compositions
    Article Snippet: .. PCR was performed with an initial 94 °C hold for 2 min, followed by 34 cycles of 94 °C for 30 s, 50 °C for 30 s, and 72 °C for 1 min 30 s, with a final extension at 72 °C for 4 min. PCR product was checked with 1% agarose gel against the GeneRuler 1 kb DNA Ladder (Thermo Scientific). .. Purify DNA fragments were purified by using DNA Clean & Concentrator (Zymo Research Corporation) and the base sequence analyzed using BioLign version 4.0.6.2 and compared with the base sequence data available in the database (GenBank) of the National Center for Biotechnology Information (NCBI).

    Article Title: CRISPR/Cas9-mediated editing of the GhJAZ2 gene improves fiber length and lint percentage in Gossypium hirsutum L
    Article Snippet: .. PCR amplification was carried out using a Bio-Rad T100 thermal cycler under the following conditions: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s. Then, the PCR product, along with a 100 bp DNA ladder (Thermo Fisher Scientific, Cat. No. SM0241), was electrophoresed on an ethidium bromide-stained 2.0% agarose gel (1× TAE buffer) at 80 V for 40 min, and visualized using a ChemiDocTM MP Imaging System (Bio-Rad). ..

    Article Title: NUP98 regulates orthoflavivirus replication through interaction with vRNA and can be targeted for antiviral purposes.
    Article Snippet: Next-generation sequencing data analysis To identify the sequences of the binding peptides, 5 μl of phages were polymerase chain reaction (PCR) amplified and barcoded using Phusion High-Fidelity PCR Master mix (Thermo Scientific). .. The amplified PCR product was confirmed on 2% agarose gel with GelRed staining using 50 bp marker (Thermo Scientific). .. Further, 25 μl of PCR product was normalized using Mag-bind Total Pure next-generation sequencing (NGS; Omega Bio-tek) and eluted with 10 μl of TE (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) buffer.

    Article Title: Resveratrol ameliorates early-weaning stressed calves via alterations in gut microbiome and metabolome.
    Article Snippet: .. The PCR product was extracted from 2% agarose gel and purified using the PCR Clean-Up Kit (YuHua, Shanghai, China) according to the manufacturer’s instructions and quantified using Qubit 4.0 (Thermo Fisher Scientific). .. Bacterial 16S rRNA Gene Library Preparation and Illumina Sequencing Purified amplicons were pooled in equimolar concentrations and paired-end sequenced (2 × 300 bp) on the Illumina MiSeq PE300 platform (Illumina, San Diego, CA).

    Article Title: Comprehensive analysis of nutrient partitioning and microbial communities in pear orchards: effects of tree age and spatial heterogeneity
    Article Snippet: .. The PCR product was extracted from a 2% agarose gel and purified using a PCR Clean-Up Kit (YuHua, Shanghai, China) according to the manufacturer’s instructions and quantified using a Qubit 4.0 (Thermo Fisher Scientific, USA). .. Purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina PE300 (Illumina, San Diego, USA) according to the standard protocols by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).

    Article Title: CRISPR/Cas9-mediated editing of the GhJAZ2 gene improves fiber length and lint percentage in Gossypium hirsutum L.
    Article Snippet: .. PCR amplification was carried out using a Bio-Rad T100 thermal cycler under the following conditions: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s. Then, the PCR product, along with a 100 bp DNA ladder (Thermo Fisher Scientific, Cat. No. SM0241), was electrophoresed on an ethidium bromide-stained 2.0% agarose gel (1× TAE buffer) at 80 V for 40 min, and visualized using a ChemiDocTM MP Imaging System (Bio-Rad). ..

    Article Title: NUP98 regulates orthoflavivirus replication through interaction with vRNA and can be targeted for antiviral purposes
    Article Snippet: To identify the sequences of the binding peptides, 5 μl of phages were polymerase chain reaction (PCR) amplified and barcoded using Phusion High-Fidelity PCR Master mix (Thermo Scientific). .. The amplified PCR product was confirmed on 2% agarose gel with GelRed staining using 50 bp marker (Thermo Scientific). .. Further, 25 μl of PCR product was normalized using Mag-bind Total Pure next-generation sequencing (NGS; Omega Bio-tek) and eluted with 10 μl of TE (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) buffer.

    Agarose Gel Electrophoresis:

    Article Title: Molecular Identification of Spirogyra Species Using rbcL Markers and Their Chemical Compositions
    Article Snippet: .. PCR was performed with an initial 94 °C hold for 2 min, followed by 34 cycles of 94 °C for 30 s, 50 °C for 30 s, and 72 °C for 1 min 30 s, with a final extension at 72 °C for 4 min. PCR product was checked with 1% agarose gel against the GeneRuler 1 kb DNA Ladder (Thermo Scientific). .. Purify DNA fragments were purified by using DNA Clean & Concentrator (Zymo Research Corporation) and the base sequence analyzed using BioLign version 4.0.6.2 and compared with the base sequence data available in the database (GenBank) of the National Center for Biotechnology Information (NCBI).

    Article Title: CRISPR/Cas9-mediated editing of the GhJAZ2 gene improves fiber length and lint percentage in Gossypium hirsutum L
    Article Snippet: .. PCR amplification was carried out using a Bio-Rad T100 thermal cycler under the following conditions: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s. Then, the PCR product, along with a 100 bp DNA ladder (Thermo Fisher Scientific, Cat. No. SM0241), was electrophoresed on an ethidium bromide-stained 2.0% agarose gel (1× TAE buffer) at 80 V for 40 min, and visualized using a ChemiDocTM MP Imaging System (Bio-Rad). ..

    Article Title: NUP98 regulates orthoflavivirus replication through interaction with vRNA and can be targeted for antiviral purposes.
    Article Snippet: Next-generation sequencing data analysis To identify the sequences of the binding peptides, 5 μl of phages were polymerase chain reaction (PCR) amplified and barcoded using Phusion High-Fidelity PCR Master mix (Thermo Scientific). .. The amplified PCR product was confirmed on 2% agarose gel with GelRed staining using 50 bp marker (Thermo Scientific). .. Further, 25 μl of PCR product was normalized using Mag-bind Total Pure next-generation sequencing (NGS; Omega Bio-tek) and eluted with 10 μl of TE (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) buffer.

    Article Title: Resveratrol ameliorates early-weaning stressed calves via alterations in gut microbiome and metabolome.
    Article Snippet: .. The PCR product was extracted from 2% agarose gel and purified using the PCR Clean-Up Kit (YuHua, Shanghai, China) according to the manufacturer’s instructions and quantified using Qubit 4.0 (Thermo Fisher Scientific). .. Bacterial 16S rRNA Gene Library Preparation and Illumina Sequencing Purified amplicons were pooled in equimolar concentrations and paired-end sequenced (2 × 300 bp) on the Illumina MiSeq PE300 platform (Illumina, San Diego, CA).

    Article Title: Comprehensive analysis of nutrient partitioning and microbial communities in pear orchards: effects of tree age and spatial heterogeneity
    Article Snippet: .. The PCR product was extracted from a 2% agarose gel and purified using a PCR Clean-Up Kit (YuHua, Shanghai, China) according to the manufacturer’s instructions and quantified using a Qubit 4.0 (Thermo Fisher Scientific, USA). .. Purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina PE300 (Illumina, San Diego, USA) according to the standard protocols by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).

    Article Title: CRISPR/Cas9-mediated editing of the GhJAZ2 gene improves fiber length and lint percentage in Gossypium hirsutum L.
    Article Snippet: .. PCR amplification was carried out using a Bio-Rad T100 thermal cycler under the following conditions: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s. Then, the PCR product, along with a 100 bp DNA ladder (Thermo Fisher Scientific, Cat. No. SM0241), was electrophoresed on an ethidium bromide-stained 2.0% agarose gel (1× TAE buffer) at 80 V for 40 min, and visualized using a ChemiDocTM MP Imaging System (Bio-Rad). ..

    Article Title: NUP98 regulates orthoflavivirus replication through interaction with vRNA and can be targeted for antiviral purposes
    Article Snippet: To identify the sequences of the binding peptides, 5 μl of phages were polymerase chain reaction (PCR) amplified and barcoded using Phusion High-Fidelity PCR Master mix (Thermo Scientific). .. The amplified PCR product was confirmed on 2% agarose gel with GelRed staining using 50 bp marker (Thermo Scientific). .. Further, 25 μl of PCR product was normalized using Mag-bind Total Pure next-generation sequencing (NGS; Omega Bio-tek) and eluted with 10 μl of TE (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) buffer.

    Amplification:

    Article Title: CRISPR/Cas9-mediated editing of the GhJAZ2 gene improves fiber length and lint percentage in Gossypium hirsutum L
    Article Snippet: .. PCR amplification was carried out using a Bio-Rad T100 thermal cycler under the following conditions: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s. Then, the PCR product, along with a 100 bp DNA ladder (Thermo Fisher Scientific, Cat. No. SM0241), was electrophoresed on an ethidium bromide-stained 2.0% agarose gel (1× TAE buffer) at 80 V for 40 min, and visualized using a ChemiDocTM MP Imaging System (Bio-Rad). ..

    Article Title: NUP98 regulates orthoflavivirus replication through interaction with vRNA and can be targeted for antiviral purposes.
    Article Snippet: Next-generation sequencing data analysis To identify the sequences of the binding peptides, 5 μl of phages were polymerase chain reaction (PCR) amplified and barcoded using Phusion High-Fidelity PCR Master mix (Thermo Scientific). .. The amplified PCR product was confirmed on 2% agarose gel with GelRed staining using 50 bp marker (Thermo Scientific). .. Further, 25 μl of PCR product was normalized using Mag-bind Total Pure next-generation sequencing (NGS; Omega Bio-tek) and eluted with 10 μl of TE (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) buffer.

    Article Title: CRISPR/Cas9-mediated editing of the GhJAZ2 gene improves fiber length and lint percentage in Gossypium hirsutum L.
    Article Snippet: .. PCR amplification was carried out using a Bio-Rad T100 thermal cycler under the following conditions: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s. Then, the PCR product, along with a 100 bp DNA ladder (Thermo Fisher Scientific, Cat. No. SM0241), was electrophoresed on an ethidium bromide-stained 2.0% agarose gel (1× TAE buffer) at 80 V for 40 min, and visualized using a ChemiDocTM MP Imaging System (Bio-Rad). ..

    Article Title: NUP98 regulates orthoflavivirus replication through interaction with vRNA and can be targeted for antiviral purposes
    Article Snippet: To identify the sequences of the binding peptides, 5 μl of phages were polymerase chain reaction (PCR) amplified and barcoded using Phusion High-Fidelity PCR Master mix (Thermo Scientific). .. The amplified PCR product was confirmed on 2% agarose gel with GelRed staining using 50 bp marker (Thermo Scientific). .. Further, 25 μl of PCR product was normalized using Mag-bind Total Pure next-generation sequencing (NGS; Omega Bio-tek) and eluted with 10 μl of TE (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) buffer.

    Imaging:

    Article Title: CRISPR/Cas9-mediated editing of the GhJAZ2 gene improves fiber length and lint percentage in Gossypium hirsutum L
    Article Snippet: .. PCR amplification was carried out using a Bio-Rad T100 thermal cycler under the following conditions: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s. Then, the PCR product, along with a 100 bp DNA ladder (Thermo Fisher Scientific, Cat. No. SM0241), was electrophoresed on an ethidium bromide-stained 2.0% agarose gel (1× TAE buffer) at 80 V for 40 min, and visualized using a ChemiDocTM MP Imaging System (Bio-Rad). ..

    Article Title: CRISPR/Cas9-mediated editing of the GhJAZ2 gene improves fiber length and lint percentage in Gossypium hirsutum L.
    Article Snippet: .. PCR amplification was carried out using a Bio-Rad T100 thermal cycler under the following conditions: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s. Then, the PCR product, along with a 100 bp DNA ladder (Thermo Fisher Scientific, Cat. No. SM0241), was electrophoresed on an ethidium bromide-stained 2.0% agarose gel (1× TAE buffer) at 80 V for 40 min, and visualized using a ChemiDocTM MP Imaging System (Bio-Rad). ..

    Staining:

    Article Title: NUP98 regulates orthoflavivirus replication through interaction with vRNA and can be targeted for antiviral purposes.
    Article Snippet: Next-generation sequencing data analysis To identify the sequences of the binding peptides, 5 μl of phages were polymerase chain reaction (PCR) amplified and barcoded using Phusion High-Fidelity PCR Master mix (Thermo Scientific). .. The amplified PCR product was confirmed on 2% agarose gel with GelRed staining using 50 bp marker (Thermo Scientific). .. Further, 25 μl of PCR product was normalized using Mag-bind Total Pure next-generation sequencing (NGS; Omega Bio-tek) and eluted with 10 μl of TE (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) buffer.

    Article Title: NUP98 regulates orthoflavivirus replication through interaction with vRNA and can be targeted for antiviral purposes
    Article Snippet: To identify the sequences of the binding peptides, 5 μl of phages were polymerase chain reaction (PCR) amplified and barcoded using Phusion High-Fidelity PCR Master mix (Thermo Scientific). .. The amplified PCR product was confirmed on 2% agarose gel with GelRed staining using 50 bp marker (Thermo Scientific). .. Further, 25 μl of PCR product was normalized using Mag-bind Total Pure next-generation sequencing (NGS; Omega Bio-tek) and eluted with 10 μl of TE (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) buffer.

    Marker:

    Article Title: NUP98 regulates orthoflavivirus replication through interaction with vRNA and can be targeted for antiviral purposes.
    Article Snippet: Next-generation sequencing data analysis To identify the sequences of the binding peptides, 5 μl of phages were polymerase chain reaction (PCR) amplified and barcoded using Phusion High-Fidelity PCR Master mix (Thermo Scientific). .. The amplified PCR product was confirmed on 2% agarose gel with GelRed staining using 50 bp marker (Thermo Scientific). .. Further, 25 μl of PCR product was normalized using Mag-bind Total Pure next-generation sequencing (NGS; Omega Bio-tek) and eluted with 10 μl of TE (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) buffer.

    Article Title: NUP98 regulates orthoflavivirus replication through interaction with vRNA and can be targeted for antiviral purposes
    Article Snippet: To identify the sequences of the binding peptides, 5 μl of phages were polymerase chain reaction (PCR) amplified and barcoded using Phusion High-Fidelity PCR Master mix (Thermo Scientific). .. The amplified PCR product was confirmed on 2% agarose gel with GelRed staining using 50 bp marker (Thermo Scientific). .. Further, 25 μl of PCR product was normalized using Mag-bind Total Pure next-generation sequencing (NGS; Omega Bio-tek) and eluted with 10 μl of TE (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) buffer.

    Purification:

    Article Title: Resveratrol ameliorates early-weaning stressed calves via alterations in gut microbiome and metabolome.
    Article Snippet: .. The PCR product was extracted from 2% agarose gel and purified using the PCR Clean-Up Kit (YuHua, Shanghai, China) according to the manufacturer’s instructions and quantified using Qubit 4.0 (Thermo Fisher Scientific). .. Bacterial 16S rRNA Gene Library Preparation and Illumina Sequencing Purified amplicons were pooled in equimolar concentrations and paired-end sequenced (2 × 300 bp) on the Illumina MiSeq PE300 platform (Illumina, San Diego, CA).

    Article Title: Comprehensive analysis of nutrient partitioning and microbial communities in pear orchards: effects of tree age and spatial heterogeneity
    Article Snippet: .. The PCR product was extracted from a 2% agarose gel and purified using a PCR Clean-Up Kit (YuHua, Shanghai, China) according to the manufacturer’s instructions and quantified using a Qubit 4.0 (Thermo Fisher Scientific, USA). .. Purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina PE300 (Illumina, San Diego, USA) according to the standard protocols by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).

    other:

    Article Title: Prime editing corrects the dilated cardiomyopathy causing RBM20-P633L-mutation in human cardiomyocytes
    Article Snippet: The solution was loaded over an iodixanol gradient (Sigma-Aldrich) of four phases (15%, 25%, 40%, and 60%) and ultracentrifuged at 200,000×g for 2 h at 18°C in a Beckman Type 70Ti rotor.



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